This study investigates the prevalence of Listeria spp in newly cutted vegetables. The influence of initial head-spaces of air – 4.9% CO2, 2.1% O2, 93% N2 and 5% CO2, 5.2% O2, 89.8% N2 – on Listeria monocytogenes and on microbial association with shredded carrots and lettuce was studied at 4ºC. These pathogens survived but did not grow in any vegetable regardless of the packaging system used. Total viable count and lactic acid bacteria and Pseudomonas were also monitored. Lactic acid bacteria were the predominant organisms in all samples. The PH dropped significantly during the storage of vegetables. The selective medium mainly used for the isolation of Listeria is oxford agar. Using isolated L.monocytogenes from vegetables, morphologic and biochemical identification was carried out. The results conferred during this study indicate the contamination of Listeria in vegetables.
L.monocytogenes, Lettuce, Listerosis
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